Energy metabolism of pregnant zebu and crossbred zebu dairy cattle.

The purpose of this study was to determine the energy partition of pregnant F1 Holstein x Gyr with average initial body weight (BW) of 515.6 kg and Gyr cows with average initial BW of 435.1 kg at 180, 210 and 240 days of gestation, obtained using respirometry. Twelve animals in two groups (six per genetic group) received a restricted diet equivalent to 1.3 times the net energy for maintenance (NEm). The proportion of gross energy intake (GEI) lost as feces did not differ between the evaluated breeds and corresponded to 28.65% on average. The daily methane production (L/d) was greater for (P<0.05) F1 HxG compared to Gyr animals. However, when expressed as L/kg dry matter (DM) or as percentage of GEI there were no differences between the groups (P>0.05). The daily loss of energy as urine (mean of 1.42 Mcal/d) did not differ (P>0.05) between groups and ranged from 3.87 to 5.35% of the GEI. The metabolizable energy intake (MEI) of F1 HxG animals was greater (P < 0.05) at all gestational stages compared to Gyr cows when expressed in Mcal/d. However, when expressed in kcal/kg of metabolic BW (BW0,75), the F1 HxG cows had MEI 11% greater (P<0.05) at 240 days of gestation and averaged 194.39 kcal/kg of BW0,75. Gyr cows showed no change in MEI over time (P>0.05), with a mean of 146.66 kcal/kg BW0. 75. The ME used by the conceptus was calculated by deducting the metabolizable energy for maintenance (MEm) from the MEI, which was obtained in a previous study using the same cows prior to becoming pregnant. The values of NEm obtained in the previous study with similar non-pregnant cows were 92.02 kcal/kg BW0.75 for F1 HxG, and 76.83 kcal/kg BW0.75 for Gyr (P = 0.06). The average ME for pregnancy (MEp) was 5.33 Mcal/d for F1 HxG and 4.46 Mcal/d for Gyr. The metabolizability ratio, averaging 0.60, was similar among the evaluated groups (P>0.05). The ME / Digestible Energy (DE) ratio differed between groups and periods evaluated (P<0.05) with a mean of 0.84. The heat increment (HI) accounted for 22.74% and 24.38% of the GEI for F1 HxG and Gyr cows, respectively. The proportion of GEI used in the basal metabolism by pregnant cows in this study represented 29.69%. However, there were no differences between the breeds and the evaluation periods and corresponded to 29.69%. The mean NE for pregnancy (NEp) was 2.76 Mcal/d and did not differ between groups and gestational stages (P>0.05).

Productive and reproductive performance of females F1 Holstein x Gir daughters of proven bulls.

Records of 234 lactations from F1 Holstein x Gyr daughters of proven bulls were used in this study for the evaluation of productive and reproductive characteristics of cows. Data were collected from a private farm located in the municipality of Santo Antônio do Monte, MG, Brazil. The objective of this study was to evaluate the effects of Holstein bull's PTA milk (predicted transmitting ability) calving year, season, and parity on the productive and reproductive characteristics of F! cows. The productive characteristics analyzed were total milk production (TMP), average daily production (ADP), days in milk (DIM), and milk yield per day of calving interval (MYDCI). The studied reproductive characteristics were service interval (SI), calving interval (CI), and gestation length (GL). The results were subjected to statistical analysis and means for significant effects were compared using Duncan's test at a significance level of 5%. F1 animals efficiently expressed their genetic potential for milk production with an average total milk production of 7842.61 kg and average daily production of 24.53 kg. Means for days in milk, milk yield per day of calving interval, service interval, calving interval, and gestation length were 322.52 days, 20.73 kg, 106.0 days, 383.23 days, and 276.18 days, respectively. Properly managed F1 Holstein x Gyr daughters of proven bulls fed a balanced diet efficiently expressed their genetic potential for milk production without adverse effects on reproduction. Moreover, Holstein x Gyr cows should be selected based on parents' PTA for milk traits and other factors such as environmental conditions and management.

Uso da somatotropina bovina recombinante (rbST) em vacas mestiças: uma revisão / Use of recombinant bovine somatotropin (rbst) in crossbred cows: review / Uso de somatotropina bovina recombinante (rbST) en vacas mestizas: una revisión

A maioria dos estudos sobre o uso da somatotropina bovina recombinante (rbST) foram conduzidos em países de clima temperado utilizando animais de genética Bos taurus e todo o protocolo de utilização foi pautado para tais animais e extrapolados aos mestiços (Bos taurus x Bos indicus) em ambientes tropicais. No entanto, existem características diferenciadas da curva de produção de leite e alguns aspectos fisiológicos que diferem os mestiços dos taurinos, sendo assim, estabelecer padrões específicos para uso do rbST para vacas mestiças podem melhorar a eficiência, reduzir custos e expandir o uso da tecnologia para os sistemas brasileiros. Estabelecer ajuste da dosagem, o intervalo, o melhor tempo para início e término da aplicação, avaliar o melhor retorno financeiro do uso para o sistema produtivo assim como as respostas produtivas e reprodutivas das vacas pode trazer adequação do uso da tecnologia no sistema de produção de gado mestiço leiteiro. O objetivo da revisão é identificar critérios a serem considerados para uso do rbST em vacas mestiças a fim de potencializar a estratégia do uso do mesmo. O rbST promove notório aumento da produção de leite e mais detalhes do protocolo de uso do hormônio para vacas mestiças necessita ser avaliado já que algumas características da curva de leite e capacidade produtiva são diferentes para animais cruzados.(AU)

Most studies on the use of recombinant bovine somatotropin (rbST) were conducted in countries with temperate climates using Bos taurus animals and the entire use protocol was based on such animals and extrapolated to crossbred animals (Bos taurus x Bos indicus) in tropical environments. However, there are different characteristics in the milk production curve and some physiological aspects that differentiate the crossbred from those cattle. Therefore, the establishment of specific standards for the use of rbST for crossbred cattle can improve efficiency, reduce costs, and expand the use of technology to Brazilian systems. Establishing dosage adjustment, the interval, the best time to start and end the application, evaluating the best financial return from use on the productive system, as well as the productive and reproductive responses of the cows can help foster the adequacy of the use of technology in the production system of crossbred dairy cattle. The purpose of this review is to identify criteria to be considered for the use of rbST in crossbred cows in order to enhance the strategy of using it. The use of rbST promotes a noticeable increase in milk production; however, further details of the hormone use protocol for crossbred cows need to be evaluated since some characteristics of the milk curve are different for crossbred animals.(AU)

La mayoría de los estudios sobre el uso de somatotropina bovina recombinante (rbST) se han realizado en países de clima templado utilizando animales de genética Bos taurus y todo el protocolo de utilización se ha pautado para tales animales y extrapolados a los mestizos (Bos taurus x Bos indicus) en ambientes tropicales. Sin embargo, existen diferentes características de la curva de producción de leche y algunos aspectos fisiológicos que diferencian al mestizo de los toros, por lo tanto, establecer estándares específicos para el uso de rbST para vacas mestizas puede mejorar la eficiencia, reducir costos y expandir el uso de tecnología para Sistemas brasileños. Establecer el ajuste de dosis, el intervalo, el mejor momento para iniciar y finalizar la aplicación, evaluar el mejor retorno económico del uso para el sistema productivo, así como las respuestas productivas y reproductivas de las vacas pueden propiciar la adecuación del uso de la tecnología en el sistema de producción de ganado mestizo lechero. El propósito de la revisión es identificar los criterios que se deben considerar para el uso de rbST en vacas mestizas con el fin de mejorar la estrategia de uso. El rbST promueve un aumento notable en la producción de leche y es necesario evaluar más detalles del protocolo de uso de hormonas para vacas cruzadas, ya que algunas características de la curva de la leche son diferentes para los animales cruzados.(AU)

Combinations of cactus pear with different roughage sources on the production, chemical composition, and milk fatty acid profile of F1 Holstein/Zebu cows.

The qualities of food, mainly of animal origin, have always been of concern to consumers. It is known that the composition of animals' diets can influence the composition of the foods produced, such as milk. Thus, the objective of this study was to evaluate diets with cactus pear in combination with different forages for F1 Holstein/Zebu cows on the chemical composition and fatty acid profile of milk. Two simultaneous 4 × 4 Latin squares were used in the experimental design. Four experimental diets were used: Diet 1, sorghum silage as the only roughage; Diet 2, sorghum silage combined with cactus pear in a proportion of 50% of the roughage (dry matter basis); Diet 3, elephant grass (Pennisetum purpureum cv. Roxo) as the only roughage; and Diet 4, elephant grass combined with cactus pear in a proportion of 50% of the roughage. The roughage:concentrate ratio was 75:25. The milk urea nitrogen was higher (16.08 mg/dL) in the milk of cows fed the diet with only elephant grass than that of milk from the other diet groups. The other analyzed variables of the chemical composition of milk were not influenced by the diets. However, there was a change in the sum of the saturated and monounsaturated fatty acids. The content of C16:0 fatty acid was higher and that of C18:0 was lower in the milk of cows fed the cactus pear diets than in that of cows fed the other diets. Higher levels of oleic acid were observed in the milk of cows fed with diets containing elephant grass than those in the milk of the cows in the sorghum forage groups. Conjugated linoleic acid (CLA) was higher in the milk of cows fed cactus pear than in that of the other cows. The combination of cactus pear with elephant grass or sorghum silage in the diet did not alter the chemical composition of milk. However, it influenced the fatty acid profile.

Nutritional efficiency of feed-restricted F1 Holstein/Zebu cows during the middle third of lactation.

OBJECTIVE: The objective of this study was to evaluate the effects of different levels of quantitative feed restriction on nutrient intake and digestibility, nitrogen balance, efficiency and feeding behavior, and productive performance in F1 Holstein/Zebu cows during the middle third of their lactation. METHODS: Sixty F1 Holstein/Zebu cows with 111.5 ± 11.75 days of lactation and an initial body weight (BW) of 499 ± 30 kg (mean ± SEM) were used. The experimental design was completely randomized with the following diet levels of feed restriction: 3.39%, 2.75%, 2.50%, 2.25% and 2.00% of BW, with 12 replications for each level.The experiment lasted for 63 days, of which each period lasted 21 days with the first 16 days for diet adaptation followed by 5 days for collection of data and samples. RESULTS: For each 1% of BW diet restriction, there was a decrease in dry matter intake of 5.26 kg/day (p &lt;0.01). There was no difference in daily milk production (p = 0.09) under the restriction levels of 3.39% to 2.0% of BW. When corrected for 3.5% fat, milk production declined (p = 0.05) 3.46 kg/day for each percentage unit of feed restriction. CONCLUSION: Restricting the feed supply for F1 Holstein/Zebu cows in the middle third of their lactation period altered nutrient intake, nitrogen balance and ingestive behavior but did not affect milk production or feed efficiency. However, considering the observed body weight loss and decrease in milk production corrected for 3.5% fat, restriction of no less than 2.5% BW is recommended.

Nutritional efficiency of feed-restricted F1 Holstein/Zebu cows in early lactation.

The quantitative feed restriction of lactating cows has been used in intensive production systems as a strategy to reduce production costs. However, the effects of this restriction in F1 Holstein/Zebu cows are unclear. The objective of this work was to evaluate the effect of quantitative feed restriction on nutrient intake and digestibility, nitrogen balance, feed efficiency, feed behavior, and productive performance in F1 Holstein/Zebu cows during early lactation. Sixty F1 Holstein × Zebu cows were used at the stage of initial lactation (50 ± 13 days of lactation), and they had an initial body weight (BW) of 482 ± 43 kg. The experimental arrangement adopted was a completely randomized design, with five feed restriction levels (3.39, 2.75, 2.50, 2.25, and 2.00% of BW) and 12 cows in each treatment group. In the short term (63 days), there were reductions of 45.9% and 47.2% in dry matter intake (P < 0.01) and crude protein (P < 0.01), respectively, when the diet supply was limited from 3.39% BW to 2.00% of BW. There were declines in intake of ether extract (P < 0.01) and nonfibrous carbohydrates (P < 0.01), but there was no change in daily milk production (P = 0.44) nor the daily milk production corrected to 3.5% fat (P = 0.12); the averages were 14.01 kg/day and 13.25 kg/day, respectively. Considering the lower body weight loss, feed restriction is recommended up to 2.5% of the BW.

Nutritional and productive parameters of Holstein/Zebu cows fed diets containing cactus pear

Objective: This study ascertained effects of cactus pear in association with different roughage in the diet of F1 Holstein/Zebu cows on intake, nutrient digestibility, nitrogen balance, ingestive behavior and performance. Methods: Eight cows with 72 ± 11 days of lactation were used. The experimental design was simultaneous in two 4 x 4 Latin squares. Four experimental diets were used: Diet 1 - sorghum silage as exclusive roughage; Diet 2 - sorghum silage associated with cactus pear in a proportion of 50% of the roughage (dry basis); Diet 3 - elephant grass (Pennisetum purpureum cv. Roxo) as exclusive roughage; Diet 4 - elephant grass associated with cactus pear in a proportion of 50% of the roughage. The roughage:concentrate ratio was 75:25. Dry matter intake (P = 0.01) was higher with sorghum silage. Results: There were differences in dry matter intake (P = 0.01), crude protein (P <0.01), ether extract (P = 0.01), non-fibrous carbohydrates (P <0.01) and total digestible nutrients (P = 0.01) among the diets. Cactus pear in the diet reduced water intake by 44.52% (P < 0.01). The nitrogen balance was 59.71% and 27.49% lower in animals treated with exclusive sorghum silage and sorghum silage associated with cactus pear in relation to diets with elephant grass and elephant grass associated with cactus pear, respectively (P < 0.01). The diets did not influence the milk production (P = 0.70), 3.5% fat corrected milk production (P = 0.72) or feed efficiency (P = 0.61). Conclusion: The association of cactus pear with sorghum or elephant grass silage does not alter milk production, reduces the intake of dry matter and water and improves the digestibility of nutrients.

Energy metabolism and partition of lactating Zebu and crossbred Zebu cows in different planes of nutrition.

The aim of this study was to determine the energy metabolism and partition of lactating Gyr and F1 Holstein x Gyr (F1 HxG) cows in different planes of nutrition. Six F1 HxG and six Gyr cows with 130 days in milking (DIM) fed corn silage and concentrate were evaluated. The experiment consisted of four periods with different levels of feeding: 1st ad libitum dry matter intake (DMI) and the others with 5, 10 and 20% restricted DMI, related to the first one. An apparent digestibility assay was performed before measurements in the respiration chamber. Total feces were collected for three days. The cows were confined for 24h in the chamber in each period to determine methane and heat production (HP). F1 HxG had higher gross energy intake (GEI), metabolisable energy intake (MEI) and digestible energy intake (DEI). GE lost in feces was higher in F1 HxG (23.7% GEI) than in Gyr (20.5%) cows. Energy lost as methane and urine was similar between the groups. The metabolisability (q) was 0.67, and the efficiency of converting ME to NE (k) was 0.56. There was no difference in the energy requirements for maintenance between breeds (426.6 MJ/kg BW0,75 average value). The energy requirements for lactation were higher in F1 HxG animals due to the higher volume of milk produced, since there was no difference in energy requirements for production of one kg of milk.

Quality of Minas fresh cheese made with milk from F1 Holstein/Zebu cows fed diets with different sources of nitrogen compounds / Qualidade do queijo Minas frescal obtido do leite de vacas F1 Holandês/Zebu alimentadas com dietas com diferentes fontes de compostos nitrogenados

This study aimed to evaluate yield, fatty acid profile, physical, chemical and sensory composition of Minas fresh cheese made with milk from cows fed diets containing different sources of nitrogen compounds (soybean meal, urea, sunflower meal and detoxified castor bean meal). Eight F1 Holstein/Zebu cows with average production of 20 kg milk corrected to 3.5% fat day-1 were distributed in two 4 × 4 Latin squares, consisting of four treatments (diets), four animals and four experimental periods. Cheese was produced on the last day of each experimental period. The physical and chemical composition, yield and texture of cheese were similar between diets. The used diets influenced the concentration of fatty acid C11:0, which was higher for diets with soybean meal and sunflower meal; C18:2 C9-T11 (CLA) was higher for diets with urea, soybean meal and sunflower meal and C20:3 for diets with soybean meal and urea. For the other saturated, monounsaturated and polyunsaturated fatty acids, differences were not detected. Different sources of nitrogen compounds in the diet for cows with average production of 20 kg milk corrected to 3.5% fat day-1 have no effect on the physical or chemical composition, yield, as well as acceptance of Minas fresh cheese. However, it can influence the fatty acid profile in the cheese fat.(AU)

Objetivou-se avaliar rendimento, perfil de ácidos graxos, composição físico-química e sensorial do queijo Minas frescal produzido do leite de vacas alimentadas com dietas com diferentes fontes de compostos nitrogenados (farelo de soja, ureia, farelo de girassol e farelo de mamona detoxicado). Foram utilizadas oito vacas F1 Holandesas/Zebu, com produção média de 20 kg de leite corrigido para 3,5% de gordura dia-1, em dois quadrados latinos 4 × 4, sendo compostos de quatro tratamentos (dietas), quatro animais e quatro períodos experimentais cada. Os queijos foram fabricados no último dia de cada período experimental. A composição físico-química, o rendimento e a textura do queijo foram semelhantes entre dietas experimentais. As dietas utilizadas influenciaram a concentração do ácido graxo C11:0, sendo superior para as dietas com farelo de soja e farelo de girassol; o C18:2 C9-T11 (CLA) mostrou-se superior para as dietas com ureia, farelo de soja e farelo de girassol; e o C20:3 para as dietas com farelo de soja e ureia. Para os demais ácidos graxos saturados, monoinsaturados e poli-insaturados não foram observadas diferenças. Diferentes fontes de compostos nitrogenados na dieta de vacas, com produção média de 20 kg de leite corrigido para 3,5% de gordura, não alteram a composição físico-química, o rendimento nem a aceitação do queijo Minas frescal, entretanto pode influenciar o perfil de ácidos graxos da gordura do queijo.(AU)

Nitrogênio não proteico em substituição ao farelo de soja na dieta de vacas F1 Holandês x Zebu / Non-protein nitrogen used in replacement of soybean meal in the diet of F1 Holstein x Zebu cows

Objetivou-se avaliar a inclusão de ureia em substituição do farelo de soja na dieta de vacas F1 Holandês x Zebu sobre parâmetros digestivos, produtivos e econômicos. Os níveis de substituição foram de 0, 33, 66 e 100%. Foram utilizadas 8 vacas distribuídas em dois quadrados latinos 4 x 4, com 4 períodos de 18 dias. As amostras de alimentos, sobras, fezes e leite foram analisadas em laboratório e a análise econômica foi realizada por custeio direto. O consumo de matéria seca, proteína bruta, fibra em detergente neutro, carboidratos não fibrosos e nutrientes digestíveis totais, assim como a digestibilidade não foram influenciados pelos níveis de ureia nas dietas. Já a digestibilidade da proteína bruta aumentou linearmente. A produção de leite, ganho de peso, escore de condição corporal e a eficiência de uso do nitrogênio dietético não foram alterados, favorecendo economicamente o uso da maior inclusão de ureia nas dietas. Concluiu-se que a substituição total do farelo de soja pela ureia na dieta de vacas primíparas F1 Holandês x Zebu, não modifica os parâmetros digestivos, mantém o escore de condição corporal, permite o uso do nitrogênio de forma eficiente e é economicamente viável em relação ao uso de farelo de soja, para uma produção média de 10 kg de leite dia.

It was evaluated the inclusion of urea in place of soybean meal in the diet of cows F1 Holstein x Zebu on digestive, productive and economic parameters. The substitution levels of soybean meal by urea were 0, 33, 66 and 100%. It was used eight cows distributed in a two Latin squares 4 x 4, with four periods of 18 days. The feed, orts, milk and feces samples were analyzed in the laboratory and the economic analysis was performed by direct costing. The DM intake, CP, NDF, NFC and TDN as well as the digestibility were not influenced by levels of urea in the diets. The CP digestibility showed increasing linear effect. Milk production, gaining weight, body condition score and the dietary nitrogen use efficiency were unchanged, favoring the use of the economically most urea inclusion in diets. It was concluded that the total replacement of soybean meal by urea in the diet of primiparous cows F1 Holstein x Zebu, does not modify the digestive parameters of animals, keeps the body condition score, allows the use of nitrogen efficiently and economically viable regarding the use of soybean meal to an average production of 10 kg of milk day

Efeito dos manejos de amansamento de primíparas no pré-parto e do maior peso vivo ao parto na rentabilidade da atividade leiteira / Effect of managements of taming primiparous during antepartum and higher body weight at calving profitability of dairy farming

Analisou-se, por meio de simulação, o efeito do uso dos manejos de amansamento de primíparas no pré-parto e do maior peso vivo ao parto na rentabilidade da atividade leiteira de um sistema de produção de leite. Durante o período chuvoso, na época do verão, as vacas foram mantidas em pastos e, durante o período da seca, foram confinadas com o uso da cana-de-açúcar in natura enriquecida com ureia e sulfato de amônio. A referência zootécnica foi do rebanho composto por vacas F1 Holandês x Gir da Fazenda Experimental de Felixlândia (FEFX) da Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), localizada no município de Felixlândia­MG, região central do estado. O inventário, bem como as despesas, as receitas e os demais dados foram cadastrados no software CUSTO BOVINO LEITE 1.0, visando obter a análise de rentabilidade. O uso dos manejos de amansamento de primíparas no pré-parto e do maior peso vivo ao parto melhora a rentabilidade de um sistema de produção de leite com vacas F1 Holandês x Gir

Analyzed by means of simulation, the effect of managements of taming primiparous during antepartum and higher body weight at calving profitability of dairy farming of milk production system. During the rainy season, in the summer season, cows were kept on pastures and, during the dry season, were confined with use of cane sugar in natura enriched with urea and ammonium sulfate. The reference zootechnical was composed herd of F1 Holstein x Gir cows of the Experimental Farm Felixlândia (FEFX) of the Agricultural Research Corporation of Minas Gerais (EPAMIG), located in the municipality of Felixlândia-MG, central region of the state. The inventory and expenditure, revenue and other data were registered on COST BOVINE MILK 1.0 software, to obtain profitability analysis. The use of managements taming primiparous during antepartum and higher body weight at calving improves the profitability of a system of milk production with F1 Holstein x Gir cows.

Effect of feeding strategies on weaning weight and milk production of Holstein × Zebu calves in dual purpose milk production systems.

The objective of this study was to evaluate the effect of five feeding strategies on calf weaning weight, and cow milk production and composition in Brazilian Holstein × Zebu cows. A total of 60 cows and their calves were allocated to each of five treatments. Cows in treatments 1, 2 and 3 were milked for 270 days and cows in treatments 4 and 5 were milked for 180 days. Calves in treatment 1 (CON) were not supplemented with concentrate whereas calves from treatment 2 (CLPN) received 1 kg of concentrate daily from 90 to 270 days of age and calves from treatment 3 received 1 kg of concentrated from 180 to 270 days of age. Calves in treatment 4 (CCPS) were supplemented with 1 kg of concentrate from 90 to 180 days of age and calves in treatment 5 (CLPS) were supplemented with 1 kg of concentrate from 90 to 270 days of age. Calves from the CLPS treatment had greater milk and protein intakes (P < 0.05) and greater growth rate than calves from the other treatments. Our results indicate that the traditional system of feeding calves with no concentrate results in a weight gain of 600 g/day. The CLPS treatment produced calves with the highest live weight and growth rate. The nutritional strategy with restricted supply of milk for the calves with concomitantly short-term concentrate supplementation does not improve performance of calves but did increase feed costs.

Efeito de níveis crescentes de ureia na alimentação de vacas sobre o rendimento, composição, perfil de ácidos graxos e sensorial do queijo minas frescal / Effect of increasing levels of urea in cows feed on yield, composition, fatty acid profile and sensory of minas fresh cheese

O estudo teve como objetivo avaliar a qualidade do queijo minas frescal processado com leite de vacas alimentadas com dietas com níveis crescentes de ureia (0; 33%; 66% e 100%, que corresponderam a 0, 0,92, 1,84 e 2,77% de PB na forma de NNP) em substituição ao farelo de soja. Foram utilizadas oito vacas F1 Holandês x Zebu, primíparas, com aproximadamente 150 dias de lactação ao início do experimento e produção média de 10kg de leite corrigido para 3,5 % de gordura dia -1 , em dois quadrados latinos 4 x 4, 4 animais, 4 dietas e 4 períodos experimentais. As amostras de leite de cada vaca, da ordenha da manhã e da tarde, foram coletadas e analisadas quanto à composição físico-química. No terceiro dia de coleta, o leite obtido de cada tratamento foi processado para fabricação do queijo minas frescal. Os produtos foram analisados quanto às suas características físico-químicas, textura, rendimento, perfil de ácidos graxos, e aceitação sensorial. Não houve efeito da adição dos níveis crescentes de ureia em substituição ao farelo de soja na dieta das vacas para todos os parâmetros que indicam a qualidade dos queijos analisados. Dessa forma, conclui-se que a substituição total da ureia pelo farelo de soja na dieta com o intuito de reduzir custo de alimentação não altera a qualidade na produção do queijo minas frescal.

The study aimed to evaluate the quality of the minas fresh cheese processed with milk cows fed diets with increasing levels of urea (0, 33%, 66% and 100%, corresponding to 0, 0.92, 1.84 and 2.77% crude protein in the form of NPN) replacing soybean meal. Eight cows F1 Holstein x Zebu, gilts, with approximately 150 days of lactation to the beginning of the experiment and average production of 10 kg of milk corrected to 3.5% fat day-1, in two Latin squares 4 x 4, 4 animals, 4 diets and 4 experimental periods. Samples of milk from each cow, the milking morning and afternoon, were collected and analyzed for physical and chemical composition. On the third day of collection, the milk obtained from each treatment was processed for the production of minas fresh cheese. Products were analyzed as to their physical and chemical characteristics, texture, yield, fatty acid profile, and sensory acceptance. There was no effect of the addition of increasing levels of urea in substitution of soybean meal in the diet of cows to all the parameters that indicate the quality of cheeses analyzed. Thus, it is concluded that the total replacement of soybean meal by urea in order to reduce power cost does not change the quality in the production of minas fresh cheese.

Characteristics of the ingestive behavior and milk production of F1 Holstein x Zebu cows fed with diets containing increasing levels of urea* / Características do comportamento ingestivo e produção de leite de vacas F1 Holandês x Zebu alimentadas com dietas contendo níveis crescentes de ureia

This work aimed to evaluate the ingestive behavior and milk production of F1 Holstein x Zebu cows subjected to increasing levels of urea in the diets. Diets contained 0, 33, 66 and 100% replacement of soybean bran by urea, which corresponded to 0, 0.92, 1.84 and 2.77% of CP in the form of NPN, formulated to be isoproteic and for an average production of 10 liters of milk per cow day-1. We used two 4 x 4 Latin squares, with 4 animals, 4 diets and 4 experimental periods of 18 days each, with the first 15 days of diet adaptation and the last three days for evaluation of ingestive behavior and milk production. The cows were subjected to visual observation at each period, for two consecutive days. There were differences in feeding and idle times, and total chewing time, with quadratic behavior for these variables. Feeding time decreased with 1.78% NPN and idle time increased with the level of 1.96% of CP in the form of NPN. The total chewing time decreased with 1.95% of CP in the form of NPN. The intake, chewing and ruminating time and the efficiencies of feeding and rumination of the DM and of the NDF did not differ. The replacement of soybean brain by urea in the diet of crossbred cows produced changes in the feeding, idle and total chewing times, however, it did not influence milk production of the cows.

Objetivou-se por meio deste trabalho avaliar o comportamento ingestivo e a produção de leite de vacas F1 Holandês x Zebu submetidas a níveis crescentes de ureia nas dietas. As dietas foram constituídas de 0, 33, 66 e 100% de substituição do farelo de soja pela ureia, o que correspondeu a 0, 0,92, 1,84 e 2,77% de PB na forma de NNP, formuladas para serem isoproteicas e para uma produção média de 10 litros de leite por vaca/dia-1. Foram utilizados dois quadrados latinos 4 x 4, com 4 animais, 4 dietas e 4 períodos experimentais de 18 dias cada, sendo os primeiros 15 dias de adaptação às dietas e os três últimos dias para avaliações do comportamento ingestivo e produção de leite. As vacas foram submetidas à observação visual a cada período, durante dois dias consecutivos. Houve diferença para os tempos de alimentação, ócio e tempo total de mastigação, com comportamento quadrático para estas variáveis. O tempo de alimentação diminuiu com 1,78% NNP e o tempo em ócio aumentou com o nível de 1,96% de PB na forma de NNP. O tempo total de mastigação diminuiu com 1,95% de PB na forma de NNP. Os tempos de consumo, mastigação e ruminação e as eficiências de alimentação e ruminação da MS e da FDN, não diferiram. A substituição do farelo de soja pela ureia na dieta de vacas mestiças ocasionou mudanças nos tempos de alimentação, ócio e tempo total de mastigação, entretanto, não influenciou a produção de leite das vacas

Characteristics of the ingestive behavior and milk production of F1 Holstein x Zebu cows fed with diets containing increasing levels of urea / Características do comportamento ingestivo e produção de leitede vacas F1 Holandês x Zebu alimentadas com dietas contendo níveis crescentes de ureia

This work aimed to evaluate the ingestive behavior and milk production of F1 Holstein x Zebu cows subjected to increasing levels of urea in the diets. Diets contained 0, 33, 66 and 100% replacement of soybean bran by urea, which corresponded to 0, 0.92, 1.84 and 2.77% of CP in the form of NPN, formulated to be isoproteic and for an average production of 10 liters of milk per cow day-1. We used two 4 x 4 Latin squares, with 4 animals, 4 diets and 4 experimental periods of 18 days each, with the first 15 days of diet adaptation and the last three days for evaluation of ingestive behavior and milk production. The cows were subjected to visual observation at each period, for two consecutive days. There were differences in feeding and idle times, and total chewing time, with quadratic behavior for these variables. Feeding time decreased with 1.78% NPN and idle time increased with the level of 1.96% of CP in the form of NPN. The total chewing time decreased with 1.95% of CP in the form of NPN. The intake, chewing and ruminating time and the efficiencies of feeding and rumination of the DM and of the NDF did not differ. The replacement of soybean brain by urea in the diet of crossbred cows produced changes in the feeding, idle and total chewing times, however, it did not influence milk production of the cows.

Objetivou-se por meio deste trabalho avaliar o comportamento ingestivo e a produção de leite de vacas F1 Holandês x Zebusubmetidas a níveis crescentes de ureia nas dietas. As dietas foram constituídas de 0, 33, 66 e 100% de substituição do farelo desoja pela ureia, o que correspondeu a 0, 0,92, 1,84 e 2,77% de PB na forma de NNP, formuladas para serem isoproteicas e parauma produção média de 10 litros de leite por vaca/dia-1. Foram utilizados dois quadrados latinos 4 x 4, com 4 animais, 4 dietas e 4períodos experimentais de 18 dias cada, sendo os primeiros 15 dias de adaptação às dietas e os três últimos dias para avaliaçõesdo comportamento ingestivo e produção de leite. As vacas foram submetidas à observação visual a cada período, durante doisdias consecutivos. Houve diferença para os tempos de alimentação, ócio e tempo total de mastigação, com comportamentoquadrático para estas variáveis. O tempo de alimentação diminuiu com 1,78% NNP e o tempo em ócio aumentou com o nívelde 1,96% de PB na forma de NNP. O tempo total de mastigação diminuiu com 1,95% de PB na forma de NNP. Os tempos deconsumo, mastigação e ruminação e as eficiências de alimentação e ruminação da MS e da FDN, não diferiram. A substituiçãodo farelo de soja pela ureia na dieta de vacas mestiças ocasionou mudanças nos tempos de alimentação, ócio e tempo total demastigação, entretanto, não influenciou a produção de leite das vacas.

Meat production in a feedlot system of Zebu-Holstein steers and heifers with dairy genetics: productive and biological analyses.

The aim of this study was to evaluate the productive and biological efficiency of steers and heifers from dairy genetics in a feedlot system in terms of meat production. Twenty-four steers and 24 heifers at 10 monthes of age, (3/4) Zebu × (1/4) Holstein were utilized. They were distributed over four feedlot times, 30, 60, 90, and 120 days with four replications for each sex, and were slaughtered at the end of each period. The productive and biological analyses were performed through comparative slaughter to determine the body composition. Heifers presented with greater intakes (P < 0.05) of dry matter in grams per kg of body weight. Steers presented with a greater (P < 0.05) final empty body weight, carcass gain, cold carcass weight, and meat proportion in the carcass; however, heifers presented with a greater subcutaneous fat thickness (P < 0.05) and, consequently, a greater (P < 0.05) fat proportion in the carcass. We conclude that steers are more efficient in their productive performance than heifers in a feedlot. For the finishing carcass fat cover, heifers need 90 days in the feedlot. The net energy requirements for maintenance are 67 kcal/EBW(0.75)/d, and the net requirements of energy (NEg) and protein (NPg) for gain can be estimated by the following equations: NEg(Mcal/d) = 0.067 × EBW(0.75) × EBG(1.095) and NPg = 162 × EBG - 5.62 × RE for the two sexes.

Características produtivas da lactação de quatro grupos genéticos F1 Holandês x Zebu / Lactation productive characteristics of four genetic groups F1 Holstein x Zebu

Estudou-se a lactação de vacas F1 Holandês x Gir, Holandês x Guzerá, Holandês x Azebuado e Holandês x Nelore em nove ordens de partos obtida em duas ordenhas diárias. Analisou-se a produção média diária, o pico de lactação, o dia que ocorreu o pico e a proporção da produção na primeira e segunda ordenha diária. O sistema de produção, no qual os animais foram criados, utilizava-se de pastagens de Brachiaria decumbens e Brachiaria brizanta como alimentação volumosa na época das chuvas e silagem de milho e ou cana-de-açúcar como suplementos na época da seca.. A ração concentrada foi fornecida de acordo com a produção leiteira. Observou que todas as características avaliadas foram diretamente influenciadas pela ordem de partos e base materna. Dentre os resultados encontrados, destaca-se que, a produção média diária de leite das vacas F1 Holandês x Gir e Holandês x Azebuado foram maiores e similares entre si, enquanto que vacas F1 Holandês x Guzerá e Holandês x Nelore tiveram menores produções e também foram similares entre si. Conclui-se que todos os grupos genéticos podem ser utilizados para a produção de leite por apresentarem produções médias diárias superiores a 10 kg/vaca/dia, sendo compatíveis com a produção de leite em pasto.

We studied the lactation of F1 Holstein x Gir, Holstein x Guzerat, Holstein x Azebuado and Holstein x Nellore cows in nine calvingorders from two daily milkings. The average daily production, the lactation peak, the day when the peak occurred and the ratio ofproduction in the first and in the second daily milking were analyzed. In the production system, in which the animals were raised, Brachiaria decumbens and Brachiaria brizanta grazing were used as roughage in the rainy season, and corn silage or cane sugaras supplement in the dry season. The concentrate ration was supplied in accordance with the production. It was observed that all of the evaluated traits were directly influenced by the calving order and maternal basis. Amongst the findings, it is outstood that, the average daily milk production of F1 Holstein x Gir and Holstein x Azebuado cows were higher and similar between them while F1 Holstein x Guzerat and Holstein x Nellore cows presented lower productions and they were also similar between them. It is concluded that all of the genotypes groups can be used for the milk production because presenting average daily production higher than 10 kg/cow/day, being compatible with milk production in pasture.

Substituição da silagem de sorgo por silagem de capim-marandu: consumo, digestibilidade e ganho de peso / Substitution of sorghum silage by marandu grass silage: intake, digestibility and weight gain

O experimento foi conduzido com o objetivo de avaliar consumo, digestibilidade e desempenho de novilhas ¾ Zebu x Holandês submetidas a diferentes níveis de substituição da silagem de sorgo por silagem de capim-marandu. Foram utilizadas vinte novihas com peso médio inicial de 346,25kg, distribuídas em delineamento experimental inteiramente casualizado, com 5 repetições, onde no Tratamento 1- fração volumosa da dieta composta por 100% de silagem de sorgo; Tratamento 2- fração volumosa da dieta composta por 70% de silagem de sorgo e 30% de silagem de capim-marandu; Tratamento 3- fração volumosa da dieta composta por 30% de silagem de sorgo e 70% de silagem de capim-marandu; Tratamento 4- fração volumosa da dieta composta por 100%de silagem de capim-marandu. Juntamente com os volumosos foi adicionado concentrado na proporção de 1,2% do peso vivo,sendo as dietas isoproteicas, tendo como única fonte de variação os diferentes volumosos e suas proporções em cada tratamento.O aumento dos níveis de substituição da silagem de sorgo por silagem de capim-marandu aumentou consumo de matéria mineral e diminuiu consumo de carboidratos não fibrosos e nutrientes digestíveis totais, porém não alterou consumo de matéria seca,proteína bruta, extrato etéreo e fibra em detergente neutro. Interferiu de forma linear decrescente nos coeficientes de digestibilidade da matéria seca, proteína bruta, fibra em detergente neutro e extrato etéreo, prejudicando o ganho de peso das novilhas.

The experiment was carried out in order to evaluate intake, digestibility and performance of ¾ Zebu x Holstein heifers submitted to different levels of substitution of sorghum silage by marandu grass silage. Twenty heifers were used with initial average weight of 346.25 kg, divided into entirely randomized experimental design with 5 repetitions in which the Treatment 1 - roughage fraction of the diet composed of 100% of sorghum silage; Treatment 2 - roughage fraction of the diet composed of 70% of sorghum silageand 30% of marandu grass silage; Treatment 3 - roughage fraction of the diet composed of 30% of sorghum silage and 70% of marandu grass silage; Treatment 4 - roughage fraction of the diet composed of 100% of marandu grass silage. The concentrate was added to roughage, made calculations for ingestion of 1.2% of the live weight, and diets isonitrogenous with the sole source of variation in the different forages and their proportions in each treatment. The increased levels of substitution of sorghum silageby marandu grass silage increased consumption of mineral matter, decreased consumption of non-fiber carbohydrates and total digestible nutrients, but did not alter the intake of dry matter, crude protein, ether extract and neutral detergent fiber. Interfered linear decrease in digestibility of dry matter, crude protein, neutral detergent fiber and ether extract, affecting weight gain of heifers.

Desempenho de novilhas Holandês na produção de embriões F1 Holandês-Zebu, em condições tropicais / Performance of Holstein heifers for F1 Holstein-Zebu embryo production, in tropical conditions

A produção in vivo de embriões bovinos é uma alternativa para a formação e manutenção de rebanhos mestiços F1 destinados à produção de leite. Objetivou-se estudar o potencial de utilização de novilhas da raça Holandês, mantidas em pasto e suplementadas, como doadoras de embrião, nas épocas de verão e inverno. Procederam-se superovulação e coleta de embriões em sete novilhas em cada época. Foram obtidos os dados climáticos, a temperatura retal e a frequência respiratória das doadoras. No verão, observou-se maior índice de temperatura e umidade (ITU) às 6h, 12h e 18h, bem como maior (P0,05) na média de estruturas recuperadas (20,14±17,47 vs. 8,57±9,40), embriões viáveis (14,57±11,91 vs. 7,14±7,24) e congeláveis (13,28±11,39 vs. 6,57±6,24) obtidos no inverno e no verão, respectivamente. Sob as condições estudadas, novilhas da raça Holandês podem ser utilizadas para a produção de embriões F1, tanto no verão, quanto no inverno.

In vivo embryo production (embryo transfer) is an alternative for F1 dairy herds formation and maintenance. The aim of this study was to evaluate the potential of utilization of grazing and supplemented Holstein heifers as embryo donors in summer or winter. Superovulation was performed in seven heifers on each season. Climatic parameters, as well as rectal temperature and respiratory rate of donors were measured. Temperature and humidity index (THI) was higher (p0.05). Holstein heifers can be used as embryo donors in both summer and winter, under the given conditions.

Desempenho de bezerros filhos de vacas F1 Holandês Zebu submetidas a diferentes sistemas de alimentação e manejo / Performance of calves from F1 Holstein x Zebu cows under different feeding systems

A viabilização de modelo de produção no qual o bezerro é componente econômico torna-se muito relevante para todo o setor produtivo. Assim, este trabalho teve como objetivo avaliar o desenvolvimento de bezerros 3/4 Zebu Holandês filhos de vacas F1 Holandês Zebu, submetidas a diferentes períodos de ordenha e alimentadas com diferentes volumosos. As 30 vacas F1 Holandês Zebu, mães dos bezerros, foram distribuídas aleatoriamente em três tratamentos: T1 – Pastagens, mais 1,250kg de proteinado por dia e ordenhadas até o final da lactação; T2 – pastagens, mais 1,250kg de proteinado por dia e ordenhadas até o 200o dia de lactação; T3 – volumoso em cocho e ordenhadas até o final da lactação. Os bezerros foram criados em piquetes, e sua alimentação constituída de leite até 90 dias de idade e como volumoso silagem de milho na época seca e pastagens no verão. Os bezerros do T2, após o 200o de lactação, permaneceram com suas mães. Nos primeiros 200 dias de lactação, período em que todas as vacas foram ordenhadas, não houve efeito significativo dos tratamentos (P>0,05) sobre os ganhos médios de peso dos bezerros. Bezerros do grupo T2, mantidos a pasto com as respectivas mães, a partir do 200º dia de lactação até a secagem definitiva das vacas, apresentaram maior ganho de peso. O manejo alternativo de ordenhar vacas até o 200o dia de lactação e posteriormente mantê-las com os bezerros até a secagem definitiva, sem que sejam ordenhadas, promove maior ganho de peso dos bezerros em relação ao manejo tradicional.

The viability of production system in which the calf is an economic component is relevant for the entire production sector. This study was carried out to evaluate the growth of ¾ HZ calves, progenies of F1 HZ cows submitted to three milking periods and fed different roughages. Thirty F1 cows were randomly allotted to three feeding treatments: T1 – Pasture plus 1.250 kg of supplement/day; T2 – Pasture plus 1.250 kg of supplement/day; T3 – Roughage offered in feedlot. The cows were milked daily to the end of the lactation period, except for cows of treatment T2, which were milked during 200 days of lactation. Calves were fed milk until 60 days of age and corn silage during the dry season and pastures in the rainy season. The T2 calves remained with their mothers from 200 days to the end of the lactation period. No difference (P>0.05) among treatments for calf weight gain until the 200 days of lactation was observed. After 200 days of lactation, T2 calves, remaining with their no milked mothers, showed higher weight gain (P<0.05) in comparison to calves of the other treatments. Calves allotted to the alternative system in which they remained with their no milked mother from 200 days of lactation to the end of lactation period show higher weight gain, in comparison to calves from the traditional system.